I piano Palazzo Farmacia, stanza 214/A

ESCCA Interlaboratory Comparison Programme: Leukemia Diagnostic (Dg), Minimal Residual Disease for B-ALL (B-ALL MRD), Minimal Residual Disease for AML (AML MRD), Minimal Residual Disease for T-ALL (T-ALL MRD) 01-September-2023 to 31-December-2023
Abilitazione e Certificazione Europea ESCCA (European society for clinical cell analysis) come operatore Citometrista conseguita il 26-6-2023
Prague School on Flow Cytometry Advanced Applications in Hematology and Immunology 16 - 19 October 2023

Citofluorimetria, Cell sorting:

Dott. Francesco is involved in research and supporting researchers in the design and implementation of their experiments using state-of-the-art flow cytometry analyzers and performing cell sorting. He is also responsible for training new users of equipment and application software. He also has strong skills in Biochemistry and Cellular Biology.

He is a contract professor in SSD BIO/13 Animal Biology Farmacia 2023-2026

He is a contract professor in SSD BIO/13 Animal Biology in STEPS 2019-2023

Ph.D. in Biochemistry and Cellular Pharmacology

KNOWLEDGMENTS: Panda classification (Peroxidase activity and nuclear density analysis (PANDA) in the diagnosis of acute leukemia, clinical oncology hematology data analysis, data interpretation of ADVIA 120 Hematology System.

Technical skills

Flow cytometry:

expertise with BD Facs Melody, Attune NxT; expertise in multiparametric flow cytometry (9 colors).
proliferation assay
cell cycle, and single-cell sorting;
Study of BRCA -1/2 mut/+ CAF isolation PDPN+ CD0+,
isolating CD24+ CD44+ CD166+ and Colorectal Cancer STEM cells.
Single cells for RNA seq for analysis of sister chromosome exchange.
CD137 assay for CD4+ and CD8+ specific activation, CD8+ exhaustion, and study of exhaustion markers in different cancer types;
Immuno Metabolism, Oxidative Stress, GSH, and ROS evaluation by Flowcytometry.
Sorting antigen-specific cells and GFP cells.
As a flow cytometry and Sorting Specialist, he is responsible for executing the different types of sorting. Its experience includes operating and performing independently, troubleshooting on cell sorters equipment, customizing the instrument to fulfill the experimental needs of the user (rare events, different shapes, and dimensions of cells), supporting panel design and multi-parametric setup instructor in the initial training on the use of instrumentation,
high-dimensional flow cytometry analysis. He is also involved in the training of new researchers and in solving scientific and technical problems that may occur during their experiments.
CD34+ ISHAGE protocol;
B/T and myeloid Leukemia Analysis using Kaluza Software.
Analisi Multi parametrica multi-dimensionale, tSNE, Flowsom ecc

Certifications:

Abilitazione e Certificazione Europea ESCCA (European society for clinical cell analysis) come operatore Citometrista conseguita il 26-6-2023
CORSO BASE DI CITOMETRIA: DAI FONDAMENTI TEORICO-STRUMENTALI ALLA PRATICA ANALITICA. Scuola Nazionale Italiana di Citometria GIC.
DIAGNOSTICA EMATOLOGICA: IL MODERNO APPROCCIO METODOLOGICO ALLA DIAGNOSTICA CITOMETRICA IN EMATOLOGIA. Scuola Nazionale italiana di citometria GIC
CITOMETRIA MULTIPARAMETRICA IN IMMUNOLOGIA: DAL FENOTIPO AI TEST FUNZIONALI. Scuola Nazionale Italiana di Citometria GIC.
CORSO DI ONCOLOGIA: L’ONCOLOGIA NELLA CITOMETRIA. DETERMINAZIONE CITOMETRICA DI EVENTI RARI: ISOLAMENTO E FENOTIPO DI CANCER STEM CELLS (CSCS), CELLULE TUMORALI CIRCOLANTI (CTCI), VESCICOLE EXTRACELLULARI (EVS), PROLIFERAZIONE E MORTE CELLULARE. Scuola Nazionale italiana di citometria GIC
EXOFLOWMETRY: LA CITOMETRIA NELLO STUDIO DELLE ACQUE NELLA MICROBIOLOGIA DEGLI ALIMENTI E NELLA FILIERA AGROALIMENTARE. Scuola Nazionale Italiana di Citometria GIC.

Attestations:

Experimental Design and Quality Control for High-Dimensional Human Immunophenotyping Studies in Large Cohorts
Mitochondrial Functionality and Metabolism in Human T Cells
Step-by-Step Multi-parameter Panel Design
Data Analysis Rigor and Reproducibility, Part 3

He is a member of ISCCA (Italian Society for Cytometric Cell Analysis) and ESCCA (European Society for Clinical Cell Analysis).

Cell sorting: BD Facs Melody,

Flow cytometry: Attune NxT and FACS Melody

Analysis software for flow cytometry: Flowjo, FCS Express, Kaluza.

Molecular biology: plasmids DNA extraction; RNA extraction and RT-PCR; PCR; nested-PCR;

Cellular biology: